A NOVEL MANUFACTURED SYSTEM DESIGNED TO ENSURE CONSISTENT CONDITIONS IN MECHANICAL PRECONDITIONING OF OVINE ADIPOSE DERIVED STEM CELLS DIFFERENTIATION TOWARDS ENDOTHELIAL CELL LINEAGE
Abstract:
Introduction: Present valvular substitutes are imperfect prostheses. Regenerative medicine imagines a viable prototype using stem cells, scaffolds and bioreactors. In our study we aimed to differentiate sheep adipose derived stem cells (ADSCs) towards endothelial cells using chemical and mechanical stimuli. For mechanical stimulation, a cooling system was imagined, adapting to the incubator’s characteristics. Materials and methods: Six ADSCs cultures were isolated, frozen and stored to-1400C. Following thawing, cells underwent an endothelial differentiation protocol composed of a static and a dynamic phase, the latter requiring a water recirculation system created by us. Results: Over 100 million ADSCs were cultured and cryopreserved. Thawing tests reported over 80% viability. Five endothelial cell cultures were obtained, confirmed by immunohistochemistry. During the dynamic differentiation the incubator’s environment was constant and stable. Conclusions: The system represents a viable option for dynamic conditioning in a normal cell culture incubator environment. We certificated it in ADSCs endothelial differentiation, replacing an expensive acquisition.
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