FOLLICULAR FLUID CONCENTRATIONS OF PHTHALATES AND THEIR METABOLITES AMONG WOMEN AFTER IN VITRO FERTILIZATION
Abstract:
The aim of the study was to identify phthalates and their metabolites from follicular fluid in patients who underwent assisted reproduction procedure (in vitro fertilization), as well as possible factors influencing their concentrations. It was demonstrated the presence and accumulation of phthalates in the follicular fluid during procedures of sampling and transportation prior to be analyzed due to the transfer of these chemicals from the medical devices used in the in vitro fertilization procedure. The developed and tested analysis methodology by gas chromatographic technique coupled with mass spectrometry allows reaching an appropriate quantification with the posibility of its application as method of toxicological diagnosis. Our study is the first in Romania to use the follicular fluid as matrix for the human biomonitoring to further explore phthalate exposure and human health consequences.
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